Reversible inhibition of DNA synthesis in hamster embryo cells in culture: action of 1,2-benzanthracene and 7,12-dimethylbenz(a)anthracene.
نویسندگان
چکیده
The effects of the relatively weak carcinogen, 1,2-benzanthracene (BA), and its potent carcinogenic derivative, 7,12dimethylbenz(a)anthracene (DMBA), on the resumption of DNA synthesis in Syrian hamster embryo cells blocked in the S phase of the mitotic cycle, were analyzed. A maximum level of binding of BA to DNA (1.08 molecule/200,000 nucleotide units) occurred after 4 hours of treatment of cells which had been released from thymidine blockage with 10—*M deoxycyti-
منابع مشابه
Protection of cultured hamster embryonic cells from 7,12-dimethylbenz(a)anthracene cytotoxicity and the induced synthesis of aryl hydroxylase.
Treatment of secondary cultures of hamster embryonic cells with an excess of thymidine (5mM) caused an inhibition of DNA synthesis which was reversible. This treatment permitted a study of the effects of polycyclic hydrocarbons on cells inhibited at the G1-S boundary and on cells entering the S phase of the cell cycle. Cytotoxic damage caused by 7,12dimethylbenz(a)anthracene (DMBA) treatment of...
متن کاملFormation of glucuronic acid conjugates of 7,12-dimethylbenz(a)anthracene phenols in 7,12-dimethylbenz(a)anthracene-treated hamster embryo cell cultures.
Secondary cultures of hamster embryo cells exposed to 0.5 nmol [G-3H]7,12-dimethylbenz(a)anthracene (DMBA) per ml medium metabolized more than 90% of the DMBA within 48 hr. Samples of medium were extracted with chloroform, methanol, and water. The chloroform phases contained about one-third of the DMBA metabolites; the major chloroform-extractable metabolite was 8,9-dihydro-8,9-dihydroxy-7,12-d...
متن کاملBenzo(a)pyrene and 7,12-dimethylbenz(a)anthracene metabolism and DNA adduct formation in primary cultures of hamster epidermal cells.
Primary cultures of hamster epidermal cells exposed to hydrocarbon, 1 microgram/ml, rapidly metabolized [3H]benzo(a)pyrene and [14C]7,12-dimethylbenz(a)anthracene to ethyl acetate:acetone- and water-soluble metabolites. By 24 hr, only 13.6% of the organic solvent-soluble radioactivity recovered in the medium was unchanged [3H]benzo(a)pyrene, and only 5.9% was unchanged [14C]7,12-dimethylbenz(a)...
متن کاملBenzo(a)pyrene and 7,12-Dimethylbenz(a)anthracene Metabolism and DMA Adduct Formation in Primary Cultures of Hamster Epidermal Cells1
Primary cultures of hamster epidermal cells exposed to hy drocarbon, 1 fig/ml, rapidly metabolized [3H]benzo(a)pyrene and [14C]7,12-dimethylbenz(a)anthracene to ethyl acetate:acetoneand water-soluble metabolites. By 24 hr, only 13.6% of the organic solvent-soluble radioactivity recovered in the medium was unchanged [3H]benzo(a)pyrene, and only 5.9% was unchanged [14C]7,12-dimethylbenz(a)anthrac...
متن کاملProtective effect of dehydroepiandrosterone against aflatoxin B1-and 7,12-dimethylbenz(a)anthracene-induced cytotoxicity and transformation in cultured cells.
Dehydroepiandrosterone, an adrenal steroid that has been reported to be produced in subnormal amounts and found in lower plasma concentrations in women with benign and malignant breast tumors, protects cultured rat liver epithelial-like cells against aflatoxin B1-induced cytotoxicity and protects hamster embryonic fibroblasts against aflatoxin B1- and 7,12-dimethylbenz(a)anthracene-induced tran...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Cancer research
دوره 28 1 شماره
صفحات -
تاریخ انتشار 1968